European Academic Research ISSN 2286-4822
ISSN-L 2286-4822
Impact Factor: 3.4546 (UIF)
DRJI Value : 5.9 (B+)
Article Details :
Article Name :
The effect of dietary methionine restriction and the mechanism of glucose and lipid metabolism in obese induced C57BL/6 Mice
Author Name :
MARIAMA MAMIE LAHAI WANG YANAN ZHANG JIAHONG GUO HAITAO GUOWEI LE1
Publisher :
Bridge Center
Article URL :
Abstract :
Background: In animal study, dietary supplementation of methionine (Met), depending on dosage is beneficial for improving several metabolic health problems, such as obesity, diabetes and cardiovascular disease which potential benefit was investigated on cardiac function in glucose and lipid metabolism. Therefore, in order to effectively complete this work, the following parameters were examined: Growth performance (Weight), fasting blood glucose levels, measurement of malondialdehyde, total antioxidant capacity levels, Creactive protein and Non-esterified Fatty Acids. Methods: In this study, 42 male C57BL/6 mice were randomly shared into three groups of 18 each and fed a diet comprises of Control (Con) group given 4% fat, 0.86% Met and 67.17 % starch. High Fat + High Met (HF+HM) group 20% fat, 0.86% Met and 51.17% starch. While High Fat + Low Met (HF+LM) received 20% fat, 0.17% Met and 51.17% starch respectively. Result: Met restriction (MetR) showed decreases in body weight gain followed by reduction in fasting blood glucose levels observed throughout the study in both Con and MetR groups. However, noticeable reduction in total antioxidant capacity (T- OAC) was observed in the HM group, but Malondialdehyde (MDA) levels were higher (?0.05) in HM than Con and MetR group suggesting oxidative stress damage. However, changes were observed in Non-Esterified Fatty Acids (NEFA) parameters in the three study groups, though not statistically significant. There was an increase in C- Reactive Protein (CRP) activity of HF+HM fed mice, which was indicative of inflammation, although not specific but found to correlate with risk of cardiovascular disease. Conclusion: Dietary MetR is effective in regulating weight gain, glucose and lipid homeostasis and restoring oxidative damage during Oxidative stress situations through adaptive changes.
Keywords :
Dietary Methionine Restriction, Oxidative Stress, Reactive Oxygen Species, Cardiovascular Disease, Essential Amino Acids

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