| Article Details : |
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| | | Article Name : | | | Genetic Variation among Ompok bimaculatus,
Clupisoma sinensis, Labeo rohita and Puntius
sophore Fishes from Jaikwadi and Vishnupuri
Dams, from Godavari River in System Using
Mitochondrial Cytochrome b Gene | | Author Name : | | | RABEEHA MANKHI JEBUR
| | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | Four species of fishes (Ompok bimaculatus, Clupisoma
sinensis, Labeo rohita and Puntius sophore) were collected from two
different location or population (Jaikwadi and Vishnupuri Dams) of
Godavari river in system , twenty samples of each species were passed
through several steps include (DNA isolation , design of specific primes
for cytochrome b gene amplification, Quantification of DNA, PCR
technical where cyt b gene amplified for Ompok bimaculatus &
Clupisoma sinensis in this study was 1066 bp long and 1252 bp long
was for (Labeo rohita and Puntius sophore) and sequencing process of
cyt b gene for each species of fishes finally analysis of sequences to
reveal genetic diversity among these species. The results showed after
sequencing process for Ompok bimaculatus & Clupisoma sinensis
species ,14 samples were analysed for Clupisoma sinensis at
Vishnupuri dam for nucleotide composition and 16 samples for Ompok
bimaculatus at Jaikwadi respectively, A (29.4%), T (U)(27.7%), G
(14.5%), C (28.5%) and A (29.0%), T(U) (26.5%), G(14.4%), C(30.1%).
nucleotide diversity, Pi(t) was 0.09309 between Nanded and Paithan
populations . Two haplotypes of Ompok bimaculatus were formed
while one haplotype of Clupisoma sinensis was formed, This indicates
represent low genetic variation of Ompok bimaculatus. where as
UPGMA cluster analysis showed sequence divergence only in Ompok bimaculatus and no divergence in Clupisoma sinensis. Unfortunately,
rest of two species (Labeo rohita and Puntius sophore) were failed to
get their cyt b gene sequenes due to some technical reasons with trying
of sequencing process. | | Keywords : | | | Cytochrome b gene, genetic diversity, haplotypes,
nucleotide composition, UPGMA & Pi(t) |
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