| Article Details : |
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| | | Article Name : | | | Comparative evaluation of serological tests for
detection of Brucella infection in Somali camels
| | Author Name : | | | AHMED A. H. KADLE1, ABDALLA M. IBRAHIM, SHADIA A. MOHAMED, ABDULKARIM A. YUSUF | | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | The comparative performance of serological methods for
diagnosing brucellosis in camels under Somali context has not been
evaluated. Thus, the objective of the present study was to compare
serological tests (Rose Bengal Plate Test RBPT, modified RBPT
mRBPT, micro-Serum Agglutination Test mSAT and competitive
Enzyme Linked Immunosorbent Assay cELISA) for detection of
Brucella antibodies in camel sera collected from camel herds reared in
Mogadishu, Somalia. The seropositivity was 1.7% by RBPT, 3.9% by
mRBPT, 3.9% by mSAT and 3.9% by cELISA. The last three tests
showed excellent agreement whereas RBPT indicated moderate
agreement. Moreover, the mRBPT performed well in the sensitivity
compared to the standard RBPT. The standard RBPT is often reported
to have a high sensitivity and recommended to use as a screening test for brucellosis control programs. However, in the current study,
mRBPT achieved incomparable sensitivity than standard RBPT and
equal sensitivity mSAT and cELISA. Therefore, the authors
recommend to use modified-RBPT to screen Brucella infection in
camels from Somalia for export and control purposes and confirm the
positive animals with competitive ELISA. | | Keywords : | | | Camel, Brucella, ELISA, mRBPT, mSAT, Somalia. |
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