| Article Details : |
|
| | | Article Name : | | | Antifungal and Anticancerous activity of 5-methylphenazine-1-carboxylic acid: Candida albicans CA
ATCC10231 - Pseudomonas aeruginosa PA01
interaction bioactive metabolite | | Author Name : | | | TIROYAONE SHIMANE TSHIKANTWA
MUHAMMAD WAJID ULLAH
XIAOHONG LI
GUANG YANG1 | | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | The significance of microbial interactions goes beyond simple
antibiosis which is commonly studied about them. A most suitable,
efficient and effective method of extracting adequate amounts a
phenazine derivative, 5-methyl-phenazine-1-carboxylic acid
(5MPCA)resulting from molecular interaction of Candida albicans CA
ATCC10231 and Pseudomonas aeruginosa PA01 in yeast potato
dextrose agar (YPDA) media, was devised. The red pigment formed on
the surface of the YPDA media in which C. albicans and P. aeruginosa
were co-cultured, was scooped and dissolved in water and syringefiltered. The extracted metabolite was purified through repeated
lyophilization and dialysis and then analyzed individually before
subsequently combining it with bacterial cellulose (BC) to form a
hybrid composite. The extracted metabolite and its composite with
bacterial cellulose exhibited antifungal activity against C. albicans
with zone of inhibition of 18 mm and 14 mm respectively. The pure
metabolite was tested for cytotoxicity against prostate cancer-3 (PC-3)
cells. The percentage cell viability of PC-3 cells declined significantly from 60% on exposure to 200µg/ml of 5MPCA to 25% at 3200µg/ml
exposure of the 5MPCA. The PC-3 cells lost their spherical morphology
as they shrinked to appear like rods. The results indicate that 5MPCA
induced apoptosis on PC-3 cell lines following mitochondrial intrinsic
pathway through the activation of caspase-3 and Bcl-2 down
regulation. Antifungal studies of the composite were also performed
and other characterizations by SEM, FTIR, TGA, XRD and WHC,
were conducted. The FTIR result indicated additional characteristic
peaks unique to 5MPCA at wavelengths of 1306.73 cm-1 and 1236.95
cm-1 which represent C-O and C-N bonds respectively, within the
5MPCA – BC hybrid. In TGA, 10% weight loss occurred in BC
composited with 0.200 w/v 5MPCA and weight loss of about 7% in
lower 5MPCA (0.100w/v) concentration within the temperature range
of 90-100oC. The 5MPCA thermogram alone was about 20% within the
same temperature range. The observed weight loss in the composite
was due to loss of water molecules adherent to the BC fiber surfaces
and molecules trapped between BC layers. Findings of this study
revealed that 5MPCA is a promising potent antifungal and
anticancerous product. Its combination with BC allows for its
application in addressing biomedical challenges like wound healing. | | Keywords : | | | Molecular interaction; metabolite, 5MPCA, Candida
albicans, Pseudomonas aeruginosa, prostate cancer-3 cells.
|
|
|
|
|
|
|
|
| Announcements
|
- Volume 9, issue 5, August issue - publishing in progress
- Volume 9, Issue 2/ May 2021 publishing IN PROGRESS
- Call for Papers: open. Articles will be uploaded constantly, once the evaluation process is complete.
- The Certificates of Publication can be downloaded from RECEIVED ARTICLES section.
- COVER - VOLUME 2, ISSUE 12 / MARCH 2015
- COVER - VOLUME 3, ISSUE 2 / MAY 2015
- COVER - VOLUME 3, ISSUE 3 / JUNE 2015
- COVER - VOLUME 3, ISSUE 4 / JULY 2015
- COVER - VOLUME 3, ISSUE 8/ NOVEMBER 2015
- COVER - VOLUME 4, ISSUE 4 / JULY 2016
- COVER - VOLUME 4, ISSUE 5 / AUGUST 2016
- COVER - VOLUME 7, ISSUE 11/ FEBRUARY 2020
- Volume 9, issue 3, June 2021 publishing in progress
|
|
|
| New Launched Project |
 |
|
|
| Recommend & Share
|
|