| Article Details : |
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| | | Article Name : | | | Design of Culture Medium and Leaf Clones are
Determinant Factors in Callus Induction of
Calendula officinalis L. | | Author Name : | | | IHAB NAZAR AL-ABASI
BASHAR ZAKI KASSAB BASHI, MOZAHIM KASIM AL-MALLAH
| | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | In this present investigation, Leaf clones of Marigold,
Calendula officinalis L., plant included four segments were used for
callus stimulation. Three different types of MS induction media were
tested. They were varied in KNO3 and in sucrose concentrations. Both
KNO3 and sucrose were increased from 1900 to 2000 mgL-1 and from
30 to 32 gL-1 respectively. Data proved that this modifications in
design of MS media enhanced callus induction. Also, the results
expressed that the different clones exhibit different response for callus
induction. C2, and C3 were the most responsive segments since they
involved few days to begin callus stimulation compared with C1 and
C4 clones. | | Keywords : | | | MS Modification, Clones type, Callus induction,
Calendula officinalis L.. |
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