European Academic Research ISSN 2286-4822
ISSN-L 2286-4822
Impact Factor: 3.4546 (UIF)
DRJI Value : 5.9 (B+)
Article Details :
Article Name :
Molecular Detection of H. Pylori from Gastric Biopsies of Dyspeptic Patients Attending Endoscopy Center, Gezira State, Sudan
Author Name :
YOSRIA MOHAMMED ELSIDDIG ALAA FAROGE IBRAHIM, ROA OSMAN ELSUMANI KOKO, OMER H. M. ARABI, MOHAMED IBRAHIM M. MALIK, ADAM DAWOUD ABAKAR, KHALID ABDELSAMEA MOHAMEDAHMED1
Publisher :
Bridge Center
Article URL :
Abstract :
Background: H. pylori are Gram negative bacteria cause most common of gastrointestinal tract infections worldwide. H. pylori can lead to serious symptomic or a symptomic illness including ulcers, gastritis, doudenitis, oesophagitis. Serious complications like gastric atrophic, mucosa-associated lymphoid tissue (MALT) lymphoma can occur. H. pylori posses cag A gene which is a virulent factor and marker for the pathogenic strain. This strain associated with greater inflammations and increased the risk of developing both peptic ulcer diseases and gastric carcinoma. Objective: The study conducted to focus on detection of H. pylori and cag A gene using polymerase chain reaction (PCR) method in biopsy samples from upper gastrointestinal diseases patients in Gezira State, Sudan. Materials and Methods: Descriptive cross - sectional study was carried out during 2016 – 2019 in Gezira State, Sudan. A total of 102 antrum biopsy samples were collected from adult male and female, their age between (20 – 70 years). Biopsy sample collected by gastroenterologists at Gezira Center for G.I.T. Endoscopy and Laparoscopic Surgery. H. pylori DNA extracted to apply the PCR technique in order to investigate H. pylori infection and cag A gene. In this study PCR for 16s rRNA accepted as gold standard method to identify the H .pylori. Results: In 102 adult dyspeptic patients (45% male, 55% female, mean of age 46.1 ± 13 years). H. pylori was detected in 53 (51.9%) biopsy samples using PCR 16s rRNA, 22 (41.5%) were positive for cag A. Epigastric pain was a common clinical feature in individuals infected with H. pylori 71 (74%), dyspepsia 28 (29.5%) and vomiting 30 (25.8%). H. pylori infection predominant in gastritis and ulcer patients. Frequency of H. pylori positive cag A was common among in ulcers, gastritis, duodenitis and esophagitis patients. Out of 102 patients 9% reported mass endoscopy finding from them 56% infected with H. pylori when tested by the PCR in those positive H. pylori 80% reported positive cag A. Conclusion: Frequency of H. pylori infection using 16s rRNA is 53 (51.9%) predominant in ulcers and gastritis patients. Cag A gene highest in gastritis and ulcers patients compare with other diseases, this gene play a role for determination the clinical outcome of H. pylori infection.
Keywords :
H. pylori, 16s rRNA, Cag A, antrum biopsy, PCR.

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