Article Details : |
| | Article Name : | | Molecular Detection of H. Pylori from Gastric
Biopsies of Dyspeptic Patients Attending
Endoscopy Center, Gezira State, Sudan | Author Name : | | YOSRIA MOHAMMED ELSIDDIG
ALAA FAROGE IBRAHIM, ROA OSMAN ELSUMANI KOKO, OMER H. M. ARABI, MOHAMED IBRAHIM M. MALIK, ADAM DAWOUD ABAKAR, KHALID ABDELSAMEA MOHAMEDAHMED1 | Publisher : | | Bridge Center | Article URL : | | | Abstract : | | Background: H. pylori are Gram negative bacteria cause
most common of gastrointestinal tract infections worldwide. H. pylori
can lead to serious symptomic or a symptomic illness including ulcers,
gastritis, doudenitis, oesophagitis. Serious complications like gastric
atrophic, mucosa-associated lymphoid tissue (MALT) lymphoma can
occur. H. pylori posses cag A gene which is a virulent factor and
marker for the pathogenic strain. This strain associated with greater inflammations and increased the risk of developing both peptic ulcer
diseases and gastric carcinoma.
Objective: The study conducted to focus on detection of H.
pylori and cag A gene using polymerase chain reaction (PCR) method
in biopsy samples from upper gastrointestinal diseases patients in
Gezira State, Sudan.
Materials and Methods: Descriptive cross - sectional study
was carried out during 2016 – 2019 in Gezira State, Sudan. A total of
102 antrum biopsy samples were collected from adult male and female,
their age between (20 – 70 years). Biopsy sample collected by
gastroenterologists at Gezira Center for G.I.T. Endoscopy and
Laparoscopic Surgery. H. pylori DNA extracted to apply the PCR
technique in order to investigate H. pylori infection and cag A gene. In
this study PCR for 16s rRNA accepted as gold standard method to
identify the H .pylori.
Results: In 102 adult dyspeptic patients (45% male, 55%
female, mean of age 46.1 ± 13 years). H. pylori was detected in 53
(51.9%) biopsy samples using PCR 16s rRNA, 22 (41.5%) were positive
for cag A. Epigastric pain was a common clinical feature in
individuals infected with H. pylori 71 (74%), dyspepsia 28 (29.5%) and
vomiting 30 (25.8%). H. pylori infection predominant in gastritis and
ulcer patients. Frequency of H. pylori positive cag A was common
among in ulcers, gastritis, duodenitis and esophagitis patients. Out of
102 patients 9% reported mass endoscopy finding from them 56%
infected with H. pylori when tested by the PCR in those positive H.
pylori 80% reported positive cag A.
Conclusion: Frequency of H. pylori infection using 16s rRNA
is 53 (51.9%) predominant in ulcers and gastritis patients. Cag A gene
highest in gastritis and ulcers patients compare with other diseases,
this gene play a role for determination the clinical outcome of H. pylori
infection. | Keywords : | | H. pylori, 16s rRNA, Cag A, antrum biopsy, PCR. |
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