European Academic Research ISSN 2286-4822
ISSN-L 2286-4822
Impact Factor: 3.4546 (UIF)
DRJI Value : 5.9 (B+)
Article Details :
Article Name :
Molecular and Immunological Detection of Toxoplasma Gondii among Sudanese Pregnant Women
Author Name :
Ammar A Abdelmola, Elameen Abd Alkareem, Aymen M Madkhali, Mohamed Elfatih Abdelwadoud
Publisher :
Bridge Center
Article URL :
Abstract :
Background: Toxoplasma gondii protozoon is widely distributed around the world and can infect all mammals and birds. While acquired toxoplasmosis is usually asymptomatic in healthy subjects, acute infection during pregnancy may lead to abortion, stillbirth, fetal neurological and ocular damages. This study aimed to determine the molecular and serological prevalence of Toxoplasma gondii and associated risk factors in women attending antenatal care in Khartoum during period 2015 to 2016. Method: This was a cross-sectional descriptive study carried out between April 2015 and October 2016 involving 200 pregnant women attending antenatal care units at selected health centers in Khartoum state Sudan to determine prevalence of toxoplasmosis among pregnant women using serological and PCR techniques. Results: The positive cases of T. Gondii recorded 26 (13%) by Molecular Technique PCR with cut off level of IgG 9.35 with significant P. value 0.0000001 using independent T test which shows Negative PCR tests were 174 (6.02471±1.35242). In contradiction with IgG antibody, IgM antibody and PCR test showed insignificant association with p value of (0.549). Conclusion: PCR is considered to be the golden standard test for Toxoplasmosis gondii. Advocating for the implementation of control and preventive measurements and routine screening services for T. gondii infection to be integrated with other antenatal care services to identify potential effects of the infection and its management should be considered as the main strategy to minimize congenital toxoplasmosis and abortion.
Keywords :
Toxoplasma gondii, Toxoplasmosis, Molecular diagnosis, IgG, IgM, PCR (Polymerase chain reaction)

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