| Article Details : |
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| | | Article Name : | | | Serum Biomarkers Panel Using High-Performance Liquid Chromatography for Early Diagnosis of Rheumatoid Arthritis in Sudanese Patients | | Author Name : | | | Mrs. Ihsan Elrashid Ahmed Abd Elsalam, Associate Professor Dr Mubarak Elsaeed Mustafa Elkarsany, Professor Dr. Nadia Madani Mohamed Ahmed, Dr. Shireen Shereen Mahy Aldin Abdallah, Dr. Mutaz Mohamed Ibrahim Ali | | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by progressive joint destruction, inflammation, and systemic complications. Early diagnosis remains a clinical challenge, particularly among seronegative patients.
Objective: This study aimed to evaluate the diagnostic utility of a novel serum biomarker panel comprising angiotensinogen (AGT), serum amyloid A-4 protein (SAA4), vitamin D-binding protein (VDBP), and retinol-binding protein 4 (RBP4) in combination with HLA Class II genotyping (HLA-DRB1 and HLA-DQB1) for improved RA detection.
Methods: A total of 200 Sudanese RA patients and two hundred age- and sex-matched controls were recruited. Serum biomarker levels were quantified using high-performance liquid chromatography (HPLC) coupled with liquid chromatography-mass spectrometry (LC-MS). HLA genotyping was performed to assess the presence of HLA-DRB1 and HLA-DQB1 alleles.
Results: Among the biomarkers, AGT demonstrated the highest diagnostic accuracy with an area under the curve (AUC) of 0.76 (P = 0.003), followed by RBP4 (AUC = 0.68, P = 0.010) and SAA4 (AUC = 0.67, P = 0.020). HLA-DRB1 positivity was significantly associated with RA (odds ratio [OR] = 1.85, P = 0.015).
Conclusion: Integrating serum biomarkers with HLA Class II genotyping enhances diagnostic accuracy for RA, particularly in seronegative cases. This combined approach may facilitate early intervention and guide personalized treatment strategies in resource-limited settings.
| | Keywords : | | | serum biomarkers panel, high-performance liquid chromatography, early diagnosis, rheumatoid arthritis, Sudanese patients |
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