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| | | Article Name : | | | Analytical Validation and Field Feasibility of a MIQE-Compliant Immune-Transcript Assay (CD3E, CD4, CD8A, IL10) from Dried Blood Spots for Integrated Classification of Pediatric Iron-Deficiency Anemia | | Author Name : | | | Momena Sami Ahmed Mohammed, Prof. Nadia Madani Mohamed Ahmed, Dr. Tarig A. M. Hamid, Dr. Shireen Shereen Mahy Aldin Abdallah, Dr. Mutaz Mohamed Ibrahim Ali | | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | Background: Iron-deficiency anemia (IDA) remains the leading pediatric hematologic disorder worldwide, compounded by infection-driven inflammation that confounds traditional iron biomarkers. Translational diagnostics increasingly seek multiplexed yet field-deployable molecular assays capable of retaining analytical rigor outside reference laboratories.
Methods: A cross-sectional study (n = 100; age 6 months | | Keywords : | | | iron-deficiency anemia; child; BRINDA; Ret-He; hepcidin; RT-qPCR; MIQE; DBS; CD3E; IL10 |
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