European Academic Research ISSN 2286-4822
ISSN-L 2286-4822
Impact Factor: 3.4546 (UIF)
DRJI Value : 5.9 (B+)
Article Details :
Article Name :
Molecular Characterization of pfmdr1 and kelch13 Variants in Plasmodium falciparum from Khartoum: Cross-Platform Validation and Targeted NGS Insights into Parasitemia Risk and Minority Variants
Author Name :
Mohammed Abdelgadir Ahmed Bashir, Prof. Mohamad Baha Eldin Ahmed Saad, Prof. Nadia Madani Mohamed Ahmed, Mrs Sarra Abdelrahman Hamad, Ibrahim Mohammed Eisa Abdelrahman, Dr. Mutaz Mohamed Ibrahim Ali
Publisher :
Bridge Center
Article URL :
Abstract :
Background: Molecular surveillance of pfmdr1 and kelch13 anchors ACT stewardship; Sanger sequencing and targeted next-generation sequencing (NGS) can expose minority variants and transmission structure that anticipate resistance spread. The objectives were to estimate the prevalence of pfmdr1 (N86Y, Y184F, D1246Y) and kelch13 key variants in outpatient falciparum malaria in Khartoum; test associations between composite mutation burden and parasitemia; (iii) quantify cross-platform concordance (RFLP and Sanger) and summarize targeted NGS metrics, minority-variant detection, and simple phylogenetic signal. Methods: Consecutive cases (n=100) underwent PCR±RFLP genotyping with bidirectional Sanger confirmation of all positives/ambiguous calls. A priori, a subset (n=25; mixed/low-density or discordant by bulk methods) received targeted NGS. Bioinformatics: Trimmomatic?BWA?GATK; haplotypes via DnaSP/Arlequin; neighbor-joining phylogeny and clade
Keywords :
pfmdr1; kelch13; ACT resistance; Sanger sequencing; targeted NGS; minority variants; parasitemia; molecular surveillance; Khartoum; falciparum malaria.
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