| Article Details : |
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| | | Article Name : | | | Comparisons detection of Chlamydia pneumoniae
by Polymerase Chain Reaction and Serology in
Khartoum state | | Author Name : | | | ALI F. KHADUM1, HANAN E. BABIKER, HUMODI A. SAEED | | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | Background: Chlamydophila pneumoniae (C. pneumoniae) is
an obligate intracellular human pathogen, causing acute and chronic
infections of the upper and lower respiratory tract. Pneumonia and
bronchitis are the most common clinical manifestations of C.
pneumoniae infection; approximately 10% - 20% of cases of
community-acquired atypical pneumoniae (CAAP) are associated with
C. pneumoniae.
Objectives: The objective of this study was to detection the
causative agent of CAAP by molecular and serological methods in
Khartoum state hospitals.
Methods: Four hundred patients with symptoms of
pneumonia were enrolled in this study. The patients attended different
Chest Units in Khartoum State. Blood and sputum were collected from
each patient. Serum was separated from blood by centrifugation while
genomic DNA was extracted from sputum specimen by bacterial
preparation kit. To detect C. pneumoniae in the two types of specimens, the former was tested by immunofluorescence test IIFT (IgM) and the
later investigated by PCR technique.
Results: Of the four hundred patients enrolled in this study
242(60.5 %) were males and 158(39.5%) females. The mean age of the
patients was 42.1 years. The disease was more prevalent among males
than females, however the relationship between C. pneumoniae and
gender was insignificant (p=0.119).there a significant relation between
C. pneumoniae and age groups (p<0.003). Adoption of
immunofluorescence test and PCR technique revealed that 43(10.75%)
and 69(17.25%) patients were infected with C. pneumoniae
respectively. The results of serological test and PCR technique were
statistically significant (p<0.000).
Conclusion: PCR the beast and accurate than conventional
serological method especially for fastidious microorganism i.e. C.
pneumoniae it is the one of the main contributing agent in Khartoum
state. | | Keywords : | | | Atypical bacterial pneumonia, Serological IIFA,
Chlamydia pneumoniae, PCR. |
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