| Article Details : |
|
| | | Article Name : | | | Investigation of Cryptosporidium Species Antigen
by ELISA Method in Stool Specimens Obtained from
patients with Diarrhoea in Kosti Teaching
Hospital, White Nile State, Sudan | | Author Name : | | | HAFIZ Y. MOHAMMED1
ABDALMONEIM M. MAGBOUL
MOHAMMED A. SULIMAN | | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | Cryptosporidium is an important parasitic protozoan causing
diarrhoea in developing and developed countries. It causes severe lifethreatening diarrhoea especially in immunosuppressed individuals.
The diagnosis of the small Cryptosporidium oocysts in stool samples by
conventional microscopy is labour intensive, time consuming and relies
on stool concentration with subsequent staining and microscopy. The
aim of this study is to evaluate the clinical utility and usefulness of
capture enzyme-linked immunosorbent assay (ELISA-IDEXX) method
in detecting Cryptosporidium oocysts in stool samples from patients
admitted with diarrhoea in Kosti Teaching Hospital, White Nile State.
In addition, specific antigen by ELISA method in stool was
investigated in order to find out whether or not it contributes to the
diagnosis of Cryptosporidium species. Stool specimens were collected
from 279 patients whose ages ranged from 4 to 85 and examined
immediately or stored frozen fresh at -65 Cº or in 10% formalin. All
specimens were examined for Cryptosporidium species antigen by
ELISA and oocysts via gold standard modified Acid Fast staining
method. 21 of the specimens were positive by modified Acid Fast
staining and by ELISA, 10 addition samples were positive by ELISA | | Keywords : | | | ELISA for Cryptosporidium antigen, Cold Acid fast
staining, Kosti, Sudan.
|
|
|
|
|
|
|
|
| Announcements
|
- Volume 9, issue 5, August issue - publishing in progress
- Volume 9, Issue 2/ May 2021 publishing IN PROGRESS
- Call for Papers: open. Articles will be uploaded constantly, once the evaluation process is complete.
- The Certificates of Publication can be downloaded from RECEIVED ARTICLES section.
- COVER - VOLUME 2, ISSUE 12 / MARCH 2015
- COVER - VOLUME 3, ISSUE 2 / MAY 2015
- COVER - VOLUME 3, ISSUE 3 / JUNE 2015
- COVER - VOLUME 3, ISSUE 4 / JULY 2015
- COVER - VOLUME 3, ISSUE 8/ NOVEMBER 2015
- COVER - VOLUME 4, ISSUE 4 / JULY 2016
- COVER - VOLUME 4, ISSUE 5 / AUGUST 2016
- COVER - VOLUME 7, ISSUE 11/ FEBRUARY 2020
- Volume 9, issue 3, June 2021 publishing in progress
|
|
|
| New Launched Project |
 |
|
|
| Recommend & Share
|
|