European Academic Research ISSN 2286-4822
ISSN-L 2286-4822
Impact Factor: 3.4546 (UIF)
DRJI Value : 5.9 (B+)
Article Details :
Article Name :
Investigation of Cryptosporidium Species Antigen by ELISA Method in Stool Specimens Obtained from patients with Diarrhoea in Kosti Teaching Hospital, White Nile State, Sudan
Author Name :
Hafiz Y. Mohammed, Abdalmoneim M. Magboul, Mohammed A. Suliman
Publisher :
Bridge Center
Article URL :
Abstract :
Cryptosporidium is an important parasitic protozoan causing diarrhoea in developing and developed countries. It causes severe life-threatening diarrhoea especially in immunosuppressed individuals. The diagnosis of the small Cryptosporidium oocysts in stool samples by conventional microscopy is labour intensive, time consuming and relies on stool concentration with subsequent staining and microscopy. The aim of this study is to evaluate the clinical utility and usefulness of capture enzyme-linked immunosorbent assay (ELISA-IDEXX) method in detecting Cryptosporidium oocysts in stool samples from patients admitted with diarrhoea in Kosti Teaching Hospital, White Nile State. In addition, specific antigen by ELISA method in stool was investigated in order to find out whether or not it contributes to the diagnosis of Cryptosporidium species. Stool specimens were collected from 279 patients whose ages ranged from 4 to 85 and examined immediately or stored frozen fresh at -65 Cº or in 10% formalin. All specimens were examined for Cryptosporidium species antigen by ELISA and oocysts via gold standard modified Acid Fast staining method. 21 of the specimens were positive by modified Acid Fast staining and by ELISA, 10 addition samples were positive by ELISA only, while 248 were negative by both methods; 65 of these negative stool samples containing 97 parasites of 10 different species. ELISA false negative was 8 and 1 false positive were observed. ELISA sensitivity was 93%, specificity was 99% and positive predictive value was 99%. Storage of specimens preserved in 10% formalin or frozen fresh at -65 Cº for up to 18 months did not appear to affect the results. There was no cross-reactivity with G. lamblia (14 negative specimens) or with the 9 other species present in the ELISA-negative stool samples. The results of ELISA indicate that it is simple, rapid, accurate, easy-to-read, reliable, sensitive and specific and standardize immunoassay test for routine diagnosis and may be useful for large-scale epidemiological studies of Cryptosporidiosis.
Keywords :
ELISA for Cryptosporidium antigen, Cold Acid fast staining, Kosti, Sudan.

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