Article Details : |
| | Article Name : | | Screening of Warburgia Ugandensis Crude Extracts
Obtained from Different Organic Solvents against
Tomato Phytophthora Infestans and Alternaria
Solani | Author Name : | | ESTHER WAITHIRA KAMAU MWORIA G.E, MAINGI J.M, MASINDE P.W1 | Publisher : | | Bridge Center | Article URL : |  | | Abstract : | | Alternaria solani and Phytophthora infestans are causative
agents of early and late blight of tomatoes respectively which are
currently controlled using fungicides. Overuse of fungicides poses
safety concerns. The research objective was to investigate the invitro
efficacy of Warburgia organic solvent crude extracts on blight
pathogens. Warburgia ugandensis stem bark sample was air dried at
room temperature then ground. The powdered material was weighed
and soaked in organic solvent then filtered and the solvent recovered
using a rotary evaporator. Multiple extraction method was used with
four organic solvents. Well diffusion method was used to screen
Warburgia extracts against A. solani and P. infestans. All assays were
performed in triplicate. Statistical analysis on inhibition zone was
carried out using analysis of variance (ANOVA). Warburgia
ugandensis hexane crude extract had the highest inhibition zone in A.
solani while methanol crude extract gave the highest mean inhibition
zone in P. infestans. All the extracts were inhibitive against P. infestans and A. solani. Further studies are required for invivo studies
and to analyze the bioactive compounds in the extracts. | Keywords : | | Warburgia ugandensis, Phytophthora infestans,
Alternaria solani, invitro inhibition, tomatoes, well diffusion method. |
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