European Academic Research ISSN 2286-4822
ISSN-L 2286-4822
Impact Factor: 3.4546 (UIF)
DRJI Value : 5.9 (B+)
Article Details :
Article Name :
Bone and Skin Tissue Engineering based on Cytotoxicity Assays of PLLA Fibrous Mats Synthesized via Centrifugal Spinning
Author Name :
Bárbara Etruri Ciocca, Nahieh Toscano Miranda, Guinea Brasil Camargo Cardoso, Ana Amélia Rodrigues, Ana Flávia Pattaro, André Luiz Jardini Munhoz, Rubens Maciel Filho
Publisher :
Bridge Center
Article URL :
Abstract :
Poly (L-lactic acid) (PLLA) is a biocompatible polymer widely used in a range of applications and is already recognized in the medical field as a biomaterial. The main objective of the present work was the production of polymeric mats to be applied in tissue engineering. PLLA mats were produced through the centrifugal spinning process. Scanning electron microscopy (SEM) analyzed the fibers diameter, disposition, and morphology. The fibers presented a uniform and constant diameter (16.24 µm), being arranged randomly. Thermogravimetric analysis (TGA) technique verified variation of mass loss with temperature, in which the material degradation temperature started at 205 °C. Differential scanning calorimetry (DSC) determined phase transition temperatures. Fourier-transform infrared spectroscopy (FT-IR) evaluated PLLA fibrous membrane chemical groups. Cytotoxicity assays verified fibroblasts and osteoblasts viabilities by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide), AlamarBlue®, and Live/Dead® for 24, 48, and 72 h. This work results demonstrated that PLLA mats have great potential for tissue engineering applications in wound healing and bone repairing, being a promising biomaterial with high biocompatibility.
Keywords :
PLLA; centrifugal spinning; tissue engineering; biomaterial; biocompatibility.

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