European Academic Research ISSN 2286-4822
ISSN-L 2286-4822
Impact Factor: 3.4546 (UIF)
DRJI Value : 5.9 (B+)
Article Details :
Article Name :
High Frequency Regeneration of Arabidopsis thaliana L. from Leaves Callus Cultures
Author Name :
RASHA FAWZI Al-JIRJEES SHIFA MAHDI SALIH1 MOZAHIM KASIM Al-MALLAH
Publisher :
Bridge Center
Article URL :
Abstract :
Arabidopsis thaliana represents a model plant, and different in vitro techniques were developed for this important plant. This study aimed at presenting an efficient plant regeneration approach from leaves derived callus. For callus initiation and regeneration leaves of 20 days- aged axenic Arabidopsis seedlings were excised, cut and cultured on both agar solidified B5 and Murashige and Skoog medium (MS) supplemented with various types and concentrations of plant growth regulators. The Results indicated that high regeneration frequency of green plantlets was observed (more than 120 shoots) from callus on B5 medium, eighty of these shoots were produced on B5 medium containing 5mgL-1BA and 0.9mgL-1IAA. The number of shoots regenerated on MS medium was 21. Regenerated shoots were easily and similarly rooted using full or 12 strength MS0 medium. The rooted plantlets were adapted and successfully transplanted in field conditions. In this study, an efficient protocol for regeneration of A. thaliana was developed and optimal concentrations of phytohormones were determined.
Keywords :
Arabidopsis thaliana, Regeneration, Callus Cultures, Leaves, Cytokinin
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