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| | | Article Name : | | | Detection of Aetiological Agents of Cervicitis and
Distribution of Selective Antimicrobial Resistance
Genes in Neisseria Gonorrhoeae | | Author Name : | | | AFSANA MAHBUB1, SUBOL CHANDRA ROY, TARIT KANTI DAS, ASOK CHANDRA DEBNATH, MD RAJIB IMRAN | | Publisher : | | | Bridge Center | | Article URL : |  | | | Abstract : | | | This cross sectional study was conducted to identify Neisseria
gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum,
Mycoplasma genitalium, Adenovirus, Herpes simplex virus-2, Human
papilloma virus and Trichomonas vaginalis from patients having
clinically suspected cervicitis attending gynecology outpatient
department of Dhaka Medical College Hospital and to detect the drug
resistance genes among the isolated N. gonorrhoeae. Chronic cervicitis
was detected by histopathology from cervical biopsy samples of VIA
positive and colposcopy positive patient. A total of 248 endocervical swab
were collected. N. gonorrhoeae were isolated and identified by Gram
stain, culture, biochemical test and polymerase chain reaction (PCR).
Out of 248 cervical swab samples, 22 (8.87%) were positive in Gram
stain, 14 (5.64%) yielded growth in culture and 26 (10.48%) were
positive by PCR for N. gonorrhoeae, 16 (6.45%) for C. trachomatis and
5(2.01%) for U. urealyticum. Wet film microscopy detected T. vaginalis
trophozoite in 11 (4.43%) cases. Among 14 culture positive N. gonorrhoeae, 100% were resistant to tetracycline and doxycycline,
92.86% were resistant to ciprofloxacin, 85.71% were resistant to
penicillin and erythromycin, 14.28% were resistant to amoxicillin and
clavulonic acid, 7.14% were resistant to azithromycin and all were
sensitive to cefixime and ceftriaxone. 71.42% of the N. gonorrhoeae
strains were identified as penicillinase producers (PPNG). Considering
culture as gold standard, the sensitivity and specificity of Gram stain
was 100% and 96.58% respectively and PCR sensitivity and specificity
was 100% and 94.88% respectively. Out of the 26 PCR positive N.
gonorrhoeae, TEM-1, gyrA and parC gene were present in 73.07%,
34.61% and 15.38% cases respectively, both gyrA and parC were present
in 42.30% cases and no TEM-135 and mosaic penA genes were found in
any of the isolated N. gonorrhoeae. The results of this study showed that
N. gonorrhoeae isolates were sensitive to ceftriaxone, cefixime and
azithromycin. PCR may be considered as a suitable method for accurate
diagnosis of gonococcal cervicitis. Multiplex PCR can be introduced for
detection of other causes of cervicitis like C. trachomatis and U.
urealyticum. Regular surveillance of antimicrobial resistance should be
done in every tertiary care hospital for detection of cephalosporin
resistant strains of N. gonorrhoeae in Bangladesh. | | Keywords : | | | Aetiological agents, Cervicitis, Antimicrobial resistance,
Neisseria Gonorrhoeae |
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